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 Thermo Scientific Immobilized GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilized on crosslinked, 6% beaded agarose.
Antibody molecules recognizing the GST portion of a recombinant fusion protein can be removed from the desired antibody by passing antiserum over an immobilized GST column. GST antibodies will bind to the immobilized GST column, whereas antibodies against other fusion proteins will pass through the column unrestrained. The expression of foreign proteins in E. coli using a glutathione S-transferase (GST) fusion system is a method of choice for studying the function of particular proteins, for purifying interacting factors and for generating antibodies against fused proteins.
Highlights:
- Provides purification of specific antibodies from those directed against
the GST tag
- Reusable - bound antibodies can be eluted with 6 M guanidinium
chloride allowing for 10 purifications with the support
References:
- Smith, D.B. and Johnson, K.S. (1988). Gene 67, 31-40.
- Bar-Peled, M. and Raikhel, N.V. (1996). Anal. Biochem. 241, 140-142.
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