Detoxi-Gel Endotoxin Removing Gel

Thermo Scientific Detoxi-Gel Endotoxin Removing Gel and Columns use Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Detoxi-Gel Endotoxin Removing Gel and quickly remove endotoxins (lipopolysaccharides or LPS) from samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be introduced.

Highlights:

• Resin uses immobilized polymixin B to remove pyrogens by binding their lipid A domains
• Reduces endotoxin levels in protein solutions, cell culture media, solutions containing pharmacological components and aqueous buffers
• 1mL of gel removes >9995 EU from a 10,000 EU challenge of LPS (greater than 99% efficiency)
• 1mL of gel binds 4000-6000 E.U. of the lipopolysaccharide from E. Coli strain 055:BS
• Protein recovery dependent upon protein type and concentration; some empirical testing required
• Stable and reusable; resin can be regenerated by stripping off the endotoxins with a 1% deoxycholate solution in pyrogen-poor water; no loss in binding capacity even after 10 regenerations

References:

1. Kohda, C., et al. (2002). Infect. Immun. 70(3), 1334-1341.
2. Wang, L., et al. (2002). J. Clin. Invest. 110(8), 1175-1184.
3. Lei, B., et al. (2002). Infect. Immun. 70(12), 6880-6890.
4. Kimoto, T., et al. (2003). Infect. Immun. 71(5), 2447-2454.
5. Madrazo, D.R., et al. (2003). Infect. Immun. 71(9), 5418-5421.
6. Goh, Y.Y., (2002). Appl. Environ. Microbiol. 68(12), 6343-6352.
7. Lavelle, E.C., et al. (2003). J. Immunol. 171, 2384-2392.
8. Ben-Menachem, G., et al. (2003). Proc. Natl. Acad. Sci. USA 100(13), 7913-7918.

Related Resources:

Detoxi-Gel Endotoxin Removing Gel FAQ

Related Products:

Pierce High Capacity Endotoxin Removal Resin
Pierce LAL Chromogenic Endotoxin Quantitation Kit