
>	2-Mercaptoethanol (BME)
>	2-Mercaptoethylamine-HCl
>	Bond-Breaker TCEP Solution, Neutral pH
>	Cysteine-HCl
>	Dithiothreitol (DTT)
>	Immobilized TCEP Disulfide Reducing Gel
>	Reduce-Imm Reducing Columns
>	TCEP-HCl

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Reducing Agents for Protein Disulfides

Purified powders, convenient solutions and solid-phase resins of disulfide reducing agents, including DTT, BME and TCEP, for stabilization of free sulfhydryls (cysteines) and reduction of disulfide bonds in peptides and proteins.


	2-Mercaptoethanol (BME) Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).


	2-Mercaptoethylamine-HCl Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.


	Bond-Breaker TCEP Solution, Neutral pH Stable, 0.5M solution of TCEP reducing agent for protein disulfide bonds; odor-free; ideal as 10X stock to make reducing SDS-PAGE sample buffers.


	Cysteine-HCl Pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1; use to make sulfhydryl assay standards or as an additive in protein refolding experiments.


	Dithiothreitol (DTT) Pure, crystalline dithiothreitol (DTT), CAS 3483-12-3; popular protein disulfide reducing agent for sample loading buffers; convenient package sizes.


	Immobilized TCEP Disulfide Reducing Gel Beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized to enable efficient reduction of peptide or protein disulfide bonds.


	Reduce-Imm Reducing Columns Beaded resin on which a thiol-based reducing agent is immobilized to enable solid-phase reduction of peptide or protein disulfide bonds; reusable.


	TCEP-HCl Pure, crystalline Tris (2-carboxyethyl) phosphine hydrochloride, CAS 5961-85-3 (TCEP), a thiol-free reductant for protein and peptide disulfide bonds.