Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.
Trypsin has a wide range of applications including amino acid analysis and protein sequencing, mapping and structural studies. Enzymes such as trypsin and chymotrypsin have become important tools in sequencing studies since they are highly selective in their cleavage of peptide bonds. Trypsin cleaves only those peptide bonds in which the carboxyl group is contributed by a lysine or an arginine residue, regardless of the length or amino acid sequence of the chain. Our Immobilized TPCK Trypsin can be substituted for free trypsin in many application and is advantageous because it minimizes autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the trypsin. Immobilized trypsin is also more stable against heat-induced denaturation, resulting in longer maintenance of activity.
Applications:
- Removal of adherent cells from tissue culture flasks
- Preparing tryptic fragments for Edman degradation sequencing
- Immobilized trypsin can be used to purify soybean trypsin inhibitor
- Use our Pierce Trypsin, MS Grade (Part No. 90057) for mass spectrometry workflows
Product Details:
Trypsin is a 23.8kDa pancreatic serine endoprotease derived from trypsinogen, an inactive precursor zymogen, after enzymatic removal of an n-terminal leader sequence by enterokinase. Once some trypsin has been formed it can catalyze the conversion of more trypsinogen into its catalytically-active form. Trypsin is treated with L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotrypsin activity without affecting trypsin activity.
| Properties of Trypsin Protease. |
| Specificity |
Cleaves specifically at the carboxyl side of arginine and lysine residues |
| Source |
Bovine pancreas |
| Molecular weight |
23,800 |
| Protease type |
Serine protease |
| Uses/applications |
Specific protein fragmentation for protein identification and sequence analysis |
| Reaction conditions |
pH 7.5-9.0, 37°C |
| Storage conditions |
Store at 4°C |
| Inhibitors |
TLCK, DFP, Aprotinin, PMSF |
| Unit definition |
One unit is equal to 1 μmole of TAME (p -toluenesulfonyl-L-arginine methyl ester) in the presence of Ca2+ hydrolyzed/minute at pH 8.2, 25°C. (One TAME unit = 57.5 National Formulatory Units). |
References:
- Cunningham, L.W. (1954) Molecular-kinetic properties of crystalline diisopropyl phosphoryl trypsin. J. Biol. Chem. 211, 13.
- Walsh, K.A. and Neurath, H. (1964) Trypsinogen and chymotrypsinogen as homologous proteins. Proc.Natl. Acad Sci. USA, 52, 884.
- Kostka, V. and Carpenter, F.H. (1964) Inhibition of chymotrypsin activity in crystalline trypsin preparations. J. Biol. Chem. 239, 1799-1803.
- Walsh, K.A. "Trypsinogens and trypsins of various species" in Methods of Enzymology, Vol. XIX, (Perlman, G. E; and Lorand, L; eds) pp. 41.
- Woolhead , C.A., et al. (2001) J. Biol. Chem. 276, 14607-14613.
- Rush, J., et al. (2005) Nature Biotech. 23, 94-101.
Related Products:
Pierce Trypsin, MS Grade (Part No. 90057)
Proteases and Digestion Kits for Mass Spectrometry
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