Heptafluorobutyric Acid (HFBA)

Thermo Scientific Pierce Heptafluorobutyric Acid (HFBA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Pierce HFBA is highly purified and stably packaged heptafluorobutyric acid that is tested and prepared for use as an ion-pairing reagent in high-pressure liquid chromatography (HPLC) methods and similar applications. The solution is tested for overall purity, chain-length purity, water content, sulfate content and absorptivity at three different UV-wavelengths. The liquid reagent is offered in convenient 1mL ampules and 100mL bottles.

Highlights:

• Greater than 99.5% purity – allows sensitive, nondestructive peptide detection at low UV wavelengths in reverse-phase HPLC protein and peptide separation systems
• High-performance packaging – HFBA packaged under nitrogen in amber glass ampules or bottles
• Economical convenience – in just a few seconds, a 1mL ampule can be used to prepare 1 liter of fresh 0.1% heptafluorobutyric acid solution for the mobile phase in reverse-phase chromatography

Applications:

• Ion pair reagent for reverse-phase HPLC
• Protein/peptide sequencing
• Protein/peptide solubilizing agent
• Solid-phase peptide synthesis
• Amino acid analysis

Product Details:

Pierce HFBA is manufactured to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

References:

1. Hermann, P.M., et al. (2000) J. Neurosci. 20, 6355-6364.
2. Hearn, M.T.W. and Hancock, W.S. (1979). Trends in Biochemical Sciences 4, N58-N62.
3. Bennett, H.P.J., et al. (1980). J. Liquid Chromatogr. 3, 1353-1365.
4. Bennett, H.P.J., et al. (1981). Biochem. 20, 4530-4538.

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Acetonitrile
Water
Amino Acid Detection Reagents