Thermo Scientific Pierce Sulfo-SHPP (water-soluble Bolton-Hunter Reagent) conjugates tyrosine-like functional groups to primary amines to increase the number of tyrosyl groups that can be iodinated by iodine-125 labeling procedures.
Highlights:
- Also known as water-soluble Bolton Hunter Reagent, sulfosuccinimidyl-3 -(4-hydroxypheynyl) propionate
- Iodinate before or after coupling Sulfo-SHPP to the molecule of interest
- Useful for introducing tyrosyl groups on proteins sensitive to small amounts of organic solvent
- Useful for labeling cell surfaces without exposing the cells to membrane permeable solvents
Product Details:
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Chemical structure of Thermo Scientific Pierce Sulfo-SHPP. Also known as water-soluble Bolton-Hunter Reagent, this compound conjugates to primary amines via its NHS-ester moiety to add tyrosine-like groups to peptides and proteins that can then be labeled with radioactive iodine (125I). For more information, see our review of NHS Ester Reaction Chemistry. |
References:
- Thompson, J.A., Lau, A.L. and Cunningham, D.D. (1987). Selective radiolabeling of cell surface proteins to a high specific activity. Biochem. 26, 743-750.
- Thirkell, D., Myles, A.D. and Russell, W.C. (1989). Serotype-8 and serocluster-specific surface-expressed antigens of Ureaplasma urealyticum. Infection and Immunity 57, 1697-1701.
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