Thermo Scientific R-Phycoerythrin is a red-fluorescent, multi-subunit protein that can be crosslinked or conjugated to other molecules to make fluorescent probes.
This fluorochrome is a member of the phycobiliprotein family of proteins isolated from the marine algae Porphyra tenera or Gastroclonium coulterii. Fluorochrome-labeled reagents have high resolution and are therefore advantageous in immunological assays, such as live cell staining, double-labeling techniques and cell sorting procedures. The strong absorption bands of the R-Phycoerythrin are in the visible region of the spectrum, extending from the green to the far-red wavelengths. The absorbance spectra extends over a broad range of potential excitation wavelengths, allowing for versatility in the excitation source and creating large Stokes shifts, thus minimizing interference from Rayleigh-scattered light.
Highlights:
- R-Phycoerythrin – red-fluorescent, multi-subunit protein in the phycobiliprotein family
- Large Stokes shift – excitation (absorption) at one of three wavelengths produces light emission at a much higher wavelength
Properties of R-Phycoerythrin:
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Alternative names
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R-Phycoerythrin, R-PE, fluorescent phycobiliprotein
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Molecular weight
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240,000
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Excitation wavelength
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480, 545, 565nm
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Emission wavelength
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578nm
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General References:
- Oi, V.T., et al. (1982). Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J. Cell Biol. 93, 981-986.
- Kronick, M.N. and Grossman, P.D. (1983). Immunoassay techniques with fluorescent phycobiliprotein conjugates. Clin. Chem. 29, 1582-1586.
- Parks, D.R. and Herzenberg, L.A. (1984). Fluorescence-activated cell sorting: theory, experimental optimization, and applications in lymphoid-cell biology. Meth. Enzymol. 108, 197-241.
- Kronick, M.N. (1986). The use of phycobiliproteins as fluorescent labels in immunoassay.
J. Immunol. Meth. 92, 1-13.
- White, J.C. and Stryer, L. (1987). Photostability studies of phycobiliprotein fluorescent labels. Anal. Biochem. 161, 442-452.
- Festin, R., et al. (1987). Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry using colloidal gold, fluorescein and phycoerythrin as labels.
J. Immunol. Meth. 101, 23-28.
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