Thermo Scientific Pierce 8M Guanidine-HCl Solution is an accurately formulated, ready-to-use solution of guanidine hydrochloride, that can be easily diluted and pH-adjusted to any concentration below 8M.
In addition to increasing solubility of hydrophobic molecules, guanidine is a general protein denaturant, unfolding proteins and altering their three-dimensional structure. Consequently, some proteins will be irreversibly altered upon interaction with guanidine solutions and may lose their binding function. Before any large-scale use of guanidine, it is best to test a small sample and determine whether the denaturing effects will adversely affect the intended use of the protein.
Highlights (of 8M Guanidine-HCl Solution):
- Any concentration below 8M guanidine can be quickly and easily prepared.
- No wasting valuable research time weighing and dissolving guanidine crystals
- Free from UV absorbing materials in the range of 225-300nm
- Free from heavy metal contaminants
- Free from degradation products such as ammonia
- Excellent stability
Applications for Guanidine Hydrochloride:
- Solubilizing proteins from inclusion bodies
- Increasing solubility of hydrophobic peptides and proteins
- Denaturing proteins
Product Details:
Chaotropes, substances that disrupt the structure of water interactions, help to solubilize hydrophobic proteins and peptides for a variety of biological purposes. For example, hydrophobic peptides and proteins may be dissolved in coupling buffer containing either 6 molar guanidine-HCl or 4 molar urea prior to immobilization on AminoLink Plus Support to create an affinity column. However, because the degradation products of guanidine and urea include ammonia, which contains a primary amine that will compete for reaction to the support, urea and guanidine solutions must be prepared with high quality reagents immediately before use.
| Properties of Guanidine Hydrochloride. |
| Alternate names |
guanidinium hydrochloride, guanidine Monohydrochloride, guanidine chloride, iminourea hydrochloride, carbamidine hydrochloride, aminomethanamidine |
| Molecular formula |
CN3H5 • HCl |
| Molecular weight |
95.54 |
| CAS Number |
50-01-1 |
| |
|
| Specifications. Pierce Guanidine-Hydrochloride is manufactured to the highest specifications to ensure the integrity of your protein sample. Each lot of Guanidine-HCl is tested to meet the following minimum specifications. |
| Guanidine HCl (Part No. 24110) |
| Purity |
> 99.5% |
| UV analysis |
A280 < 0.010 |
| (6 M solution) |
A260 < 0.060 |
| Copper (Cu) |
<1 ppm |
| Iron (Fe) |
<1 ppm |
| Lead (Pb) |
<1 ppm |
| Zinc (Zn) |
<1 ppm
|
| 8M Guanidine HCl Solution (Part No. 24115) |
| Density |
1.183 - 1.188 |
| UV analysis |
A280 < 0.02 |
| |
A260 < 0.03 |
| UV cutoff |
219 + 2 nm |
| Guanidine Hydrochloride Molarity Table. Beginning with 10 ml of 8 Molar Guanidine Hydrochloride Solution, dilution to the indicated final volume will result in the stated molarity. |
|
Molarity
|
Final Volume
|
Molarity
|
Final Volume
|
|
8 M
|
10 ml
|
3 M
|
26.7 ml
|
|
7 M
|
11.4 ml
|
2 M
|
40 ml
|
|
6 M
|
13.3 ml
|
1.5 M
|
52 ml
|
|
5 M
|
16 ml
|
1 M
|
80 ml
|
|
4 M
|
20 ml
|
0.5 M
|
160 ml
|
References:
- Tanaka, S., et al. (1985) J. Biochem.97, 1377-1384.
- Wong, K.P., et al. (1971) J. Biochem.40, 459-464.
Related Products:
Other denaturants and chaotropes
Inclusion Body Solubilization Reagent
Protein Refolding Kit
|
