Thermo Scientific Pierce Ethylenediamine Dihydrochloride is a simple compound that can be used with EDC (carbodiimide) crosslinker to polymerize or amine-modify carboxylate molecules for a variety of applications.
Ethylenediamine Dihydrochloride is composed of two amines separated by a short hydrocarbon spacer. This versitle reagent is used for a variety of applications, including covalent modification of carboxylic acids to block the carboxylates and form terminal amino groups. Such amino groups can then be used for a variety of conjugation strategies. Furthermore, oligonucleotides can be amine-modified for labeling with biotin or fluorophores or immobilized onto a solid support using amine conjugation chemistries (see Tech Tip # 30).
- Amine-modify carboxylic acids using EDC
- Amine-modify aldehyde groups by reductive amination
- Amine-modify oligonucleotides using ethylenediamine, EDC and imidazole
- Chemical name: Ethylenediamine Dihydrochloride
- Synonyms: 1,2-Ethanediamine; 1,2-Diaminoethane
- Chemical formula: C2H8N2•2HCl
- Molecular weight: 133.02
- CAS Number: 107-15-3
- Form: Crystals, free of foreign matter
- Purity: Greater than 98%
- Hermanson, G.T. (2008). Bioconjugate Techniques, 2nd ed., Elsevier Inc., pp. 114-118, 125. (Part No.20036)
EDC – A carbodiimide crosslinker for zero-length, carboxyl-to-amine conjugation