SPB

Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.

Highlights:

• DNA intercalating agent and nucleic acid probe^1,2
• Reagent for photo-immobilization to microplates³
• Melting point: 177-178°C
• Reactive groups: NHS ester and psoralen
• Reactive toward: amino groups and thymine

Product Details:

References:

1. Laskin, J.D., et al. (1986). Psoralen potentiate ultraviolet light induced inhibition of epidermal growth factor binding. Proc. Natl. Acad. Sci. USA 83, 8211.
2. Inman, R.B. and Schnos, M. (1987). Electron microscopic identification of supercoiled regions ina complex DNA structure. J. Mol. Biol. 193, 377.
3. Elsner, H.I. and Mouritsen, S. (1994). Use of psoralens for covalent immobilization of biomolecules in solid phase assays. Bioconjugate Chem. 5, 463-467.