Thermo Scientific Pierce MMTS (methyl methanethiosulfonate) is a small compound that reversibly blocks cysteines and other sulfhydryl groups, enabling the study of enzyme activation and other protein functions.
MMTS (methyl methanethiosulfonate) is a sulfhydryl-reactive compound that can reversibly sulfenylate thiol-containing molecules. Reacting MMTS with reduced sulfhydryls (-SH) results in their modification to dithiomethane (-S-S-CH3). Treatment with reducing agents, such as dithiothreitol (DTT), 2-mercaptoethanol (2-ME) or Tris(2-carboxyethyl) phosphine hydrochloride (TCEP), will cleave the disulfide groups to restore the original sulfhydryl. MMTS is commonly used to study biochemical pathways involving thiol-dependent enzymes.
- Converts sulfhydryl groups on cysteine side chains into -S-S-CH3
- Reaction is reversible with DTT or TCEP, restoring the free sulfhydryl
- Used to modify thiol groups in creatine kinase
- Chemical name: Methyl methanethiosulfonate
- Reactive toward: Sulfhydryl groups
- Chemical formula: C2H6O2S2
- CAS number: 2949-92-0
- Molecular weight: 126.20
- Form: Liquid
- Boiling point: 85-87°C/0.8mm
- Kirley, T.L. (1989). Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analyses. Anal Biochem 180:231.
- Smith, D.J., et al. (1975). Simple alkanethiol groups for temporary blocking of enzymes. Biochemistry 14:766.
Ellman's Reagent - assay reagent for measuring free sulfhydryls
Bioconjugate Techniques, Second Edition