Thermo Scientific Pierce Sodium meta-Periodate is a gentle oxidizing agent that cleaves cis-diols in carbohydrate sugars to create amine-reactive aldehydes, providing many uses relating to the study and detection of glycoproteins.
Sodium meta-periodate converts cis-glycol groups in carbohydrates to amine-reactive aldehyde groups. Carbohydrate groups in glycoproteins are excellent sites for modification or crosslinking reactions because they allow the conjugation reaction to be directed away from amino acids in the polypeptide chain that could be critical for protein activity. Sodium meta-periodate cleaves bonds between adjacent carbon atoms that contain hydroxyl groups (cis-glycols), creating two aldehyde groups that are spontaneously reactive to amine- and hydrazide-activated labeling, immobilization supports and crosslinking reagents.
- Immobilize glycoproteins to a hydrazide-activated solid support
- Conjugate antibodies to enzymes, such as horseradish peroxidase, to produce a detection antibody
- Probe for cell-surface polysaccharides
- Detect carbohydrate-containing proteins using hydrazide-containing probes.
- Chemical name: Sodium meta-periodate
- Alternative names: Periodic acid, sodium salt
- Chemical formula: NaIO4
- Molecular weight: 213.89
- Form: White crystalline solid, free-flowing, free of foreign material
- Hermanson, G.T. (2008). Bioconjugate Techniques, Second Edition, Academic Press. pp. 130-131.
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