Thermo Scientific Pierce Hydroxylamine-HCl has many uses, which includes cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation.
Hydroxylamine•HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine•HCl.
- Exposes protected sulfhydryl groups on SATA-modified molecules
- Cleaves carbonyl-containing crosslinkers such as EGS
- Chemical name: Hydroxylamine Hydrochloride
- Chemical formula: NH2OH•HCl
- Molecular weight: 69.49
- CAS Number: 5470-11-1
- Form: White crystals, free of foreign material
- Solubility: 10%
- Abdella, P., et al. (1979). A new cleavable reagent for cross-linking and reversible immobilization of proteins. Biochem Biophys Res Commun 87(3):732-42.
- Duncan, R. J. S., et al. (1983). A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay. Anal Biochem 132:68-73.
SATA – adds a protected sulfhydryl to a primary amine
SATP – slightly longer version of SATA
EGS – long-arm hydroxylamine-cleavable crosslinker
Sulfo-EGS – water-soluble analog of EGS