Effective reagent for modifying and detecting arginine residues.
Thermo Scientific Pierce HPG (p-hydroxyphenylglyoxal) reacts to specifically modify arginine residues under mild conditions to yield spectrophotometrically measurable signal for amino acid detection.
Highlights:
Arginine-specific – reacts specifically with arginine residues under mild conditions (pH 7 to 9, 25°C)
Quantitative – reaction follows Beer's Law at 5 to 50µM and can be monitored at 340nm (pH 9)
Superior to alternatives – more resistant to oxidation than p-nitrophenylglyoxal and more water-soluble than phenylglyoxal
Reagent Properties:
Alternative names: HPG monohydrate
Chemical name: (p-Hydroxyphenyl)glyoxal; (4-Hydroxyphenyl)(oxo)acetaldehyde
Reactive toward: Arginine (Arg, R)
Chemical formula: C8H6O3
CAS number: 197447-05-5; 24645-80-5
Molecular weight: 150.13 (supplied as monohydrate: 168.15)
Form: Off-white crystalline powder
Product Details:
Chemical structure of p-hydroxphenylglyoxal (HPG).
References:
Yamasaki, R.B., et al. (1980). Anal. Biochem.109, 32-40.
Ordering Information
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Pkg. Size
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Price
20100
HPG (p-Hydroxyphenylglyoxal)
Formulation: p-Hydroxyphenylglyoxal; crystalline powder (at least 85% pure) Sufficient For: Modifying approx. 1mg peptide per 1mg reagent (100mg total)
