Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.
Sulfo-LC-SPDP is Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.
Highlights:
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343nm, the reaction can be easily followed
- Water soluble, amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50mM DTT or TCEP at pH 8.5
- Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
- Cleavable crosslinker allows separation of crosslinked products
- Sulfo-LC-SPDP crosslinker is not membrane permeable, so crosslinking can be carried out on the cell surface
Applications:
- Reversible crosslinking of proteins
- Adding sulfhydryl groups to proteins by attachment to lysine residues
- Protein crosslinking to create antibody-enzyme and other bioconjugates via a two-step reaction
- Attaching haptens to carrier proteins for antibody production
- Preparing immunotoxin conjugates
Product Details:
![Sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate; Sulfo-LC-SPDP](/images/structures/21650-Sulfo-LC-SPDP-stc.jpg "Sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate; Sulfo-LC-SPDP")
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Properties of Sulfo-LC-SPDP.
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Molecular formula
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C18H22O8N3S3Na
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Molecular weight
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527.57
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Spacer arm length
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15.7 Å
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Storage conditions
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-20°C, protect from moisture, use only fresh solutions
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Reactive groups
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NHS ester, reacts with primary amines
Pyridyl disulfide, reacts with sulfhydryls
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Specifications:
We manufacture Sulfo-LC-SPDP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-LC-SPDP is tested to meet the following minimum specifications.
Identity IR scan shows only peaks characteristic of the structure and functional groups of Sulfo-LC-SPDP crosslinker. Solubility is at least 95 mg/ml in DI water. Purity is greater than 90% by quantitative NMR (the highest standard for crosslinker purity).
References:
- Carlsson, J., et al. (1978) Biochem. J.173, 723-737.
- Stan, R.V. (2004) Am. J. Physio.l Heart Circ. Physiol.286, H1347-H1353.
- Mader, C., et al. (2004) J. Bacteriol.186, 1758-1768.
Related Products:
LC-SPDP
PEG4-SPDP
PEG12-SPDP
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