A water-soluble amine-to-sulfhydryl crosslinker with a long spacer arm (16.3 angstroms).
Thermo Scientific Pierce Sulfo-KMUS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (16.3 angstroms).
Long aliphatic spacer arm
Non-cleavable extended aliphatic cross-bridge
Water-soluble with enhanced sphere of coupling
Sulfo-NHS ester reacts with primary amines at pH 7-9, forming a stable amide bond
Maleimide reacts with -SH groups at pH 6.5-7.5, forming a stable thioether bond
Reactive groups: sulfo-NHS ester and maleimide
Reactive toward: sulfhydryl and amino groups
Fujiwara, K., et al. (1988). Sandwich enzyme immunoassay of tumor-associa- ted antigen sialosylated Lewis (x) using ß-D-galactosidase coupled to a monoclonal antibody of IgM isotype. J. Immunol. Methods.112, 77-83.
See also: Sulfo-GMBS, Sulfo-EMCS.
Formulation: N-kappa-Maleimidoundecanoyl-oxysulfosuccinimide ester; solid Sufficient For: 104mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use