A water-soluble amine-to-sulfhydryl crosslinker with a medium-length spacer arm (9.4 angstroms).
Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).
Sulfonated analog of EMCS has improved solubility in aqueous buffer systems
Sulfo-NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
Increased sphere of coupling vs. Sulfo-GMBS
Aliphatic spacer offers low potential for eliciting an immune response
Reactive groups: maleimide and Sulfo-NHS ester
Reactive toward: sulfhydryl and amino groups
Fujiwara, K., et al. (1988). J. Immunol. Methods112, 77-83.
Peeters, J.M., et al. (1989). J. Immunol. Methods120, 133-143.
Hermanson, G.T. (1996). Bioconjugate Techniques, San Diego: Academic Press,
pp. 243-245. (EMCS use can be modeled after GMBS.)
Formulation: N-epsilon-Maleimidocaproyl-oxysulfosuccinimide ester; powder solid Sufficient For: 122mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use