A long-chain disulfide crosslinker for strong yet reversible amine-to-sulfhydryl conjugation.
Thermo Scientific Pierce SMPT is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form strong (hindered) yet cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
Contains a hindered disulfide bond; has formed immunotoxins with improved stability
In vitro, an SMPT conjugate was as effective as conjugates formed with SPDP and 2-IT
Does not require exposing the antibody to reducing agents
Reactive groups: NHS ester and pyridyldithio
Reactive towards: amino and sulfhydryl groups
Ghetie, V., et al. (1990). Bioconjugate Chem. 1, 24-31.
Formulation: 4-Succinimidyloxycarbonyl-alpha-methyl-alpha(2-pyridyldithio)toluene; powder solid Sufficient For: 129mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use