SMPH

Thermo Scientific Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 angstroms).

Highlights:

• Amine- and sulfhydryl-reactive heterobifunctional crosslinker
• NHS ester reacts with primary amines at pH 7-9, forming a stable amide bond
• Maleimide reacts with -SH groups at pH 6.5-7.5, forming a stable thioether bond
• Non-cleavable and water-insoluble
• More hydrophyllic character to the cross-bridge than BMPS, GMBS, EMCS or SMPB
• Reactive groups: maleimide and NHS ester
• Reactive toward: sulfhydryl and amino groups

Product Details:

References:

1. Thorpe, P.E., et al. (1984). Blockade of the galactose-binding sites of ricin by its linkage to antibody. Specific cytotoxic effects of the conjugates. Eur. J. Biochem 140, 63-71.
2. Rector, E.S., Schwenk, R.J., Tse, K.S. and Sehon, A.H. (1978). A method for the preparation of protein-protein conjugates of predetermined composition. J. Immuno. Methods 24, 321-336.