An amine-to-sulfhydryl crosslinker with a medium-length aromatic spacer arm (11.6 angstroms).
Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).
Extended chain analog of MBS
Conjugates formed with SMPB were shown to be more stable in serum than SPDP conjugates
Reactive groups: NHS ester and maleimide
Reactive towards: amino and sulfhydryl groups
Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15), 3031-3039.
Iwai, K., Fukuoka, S.-I., Fushiki, T., Kido, K., Sengoku, Y. and Semba, T. (1988). Preparation of a verifiable peptide-protein immunogen: direction-controlled conjugation of a synthetic fragment of the monitor peptide with myoglobin and application for sequence analysis. Anal. Biochem. 171, 277-282.
Formulation: Succinimidyl 4-(p-maleimidophenyl)butyrate; powder solid Sufficient For: 140mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use