SBAP

Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.

Highlights:

• Useful for preparing cyclic peptides and peptide conjugates
• NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
• Bromacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bonds
• Spacer maintains peptide-like character in the crosslinked species
• Resulting crosslink is susceptible to acid hydrolysis
• Reactive groups: NHS ester and bromacetyl
• Reactive toward: amino and sulfhydryl groups

Product Details:

References:

1. Inman, J.K., Highet, P.F., Kolodny, N. and Robey, F.A. (1991). Synthesis of N-alpha-(tertbutoxycarbonyl)-N-epsilon-[N-(bromoacetyl)-beta-alanyl]-L-lysine: Its use in peptide synthesis for placing a bromoacetyl crosslinking function at any desired sequence position. Bioconjugate Chem. 2, 458-463.