A short, NHS-ester and bromoacetyl crosslinker for amine-to-sulfhydryl conjugation.
Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.
Useful for preparing cyclic peptides and peptide conjugates
NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
Bromacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bonds
Spacer maintains peptide-like character in the crosslinked species
Resulting crosslink is susceptible to acid hydrolysis
Reactive groups: NHS ester and bromacetyl
Reactive toward: amino and sulfhydryl groups
Inman, J.K., Highet, P.F., Kolodny, N. and Robey, F.A. (1991). Synthesis of N-alpha-(tertbutoxycarbonyl)-N-epsilon-[N-(bromoacetyl)-beta-alanyl]-L-lysine: Its use in peptide synthesis for placing a bromoacetyl crosslinking function at any desired sequence position. Bioconjugate Chem.2, 458-463.
Formulation: Succinimidyl 3-(bromoacetamido)propionate; solid Sufficient For: 163mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use