Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Highlights:
- Pyridyldisulfide group provides a cleavable disulfide bond
- Oxidized carbohydrate-reactive hydrazide
- Reactive groups: pyridyldisulfide and hydrazide
- Reactive toward: sulfhydryl groups and carbohydrate
- Use with EDC for reactivity toward carboxyl groups
Product Details:
Related Resources:
A guide to crosslinking and protein modification
An overview of glycosylation and methods to analyze glycoproteins and glycopeptides
References:
- Friden, P.M., et al. (1993). Science 259, 373-377.
- Zara, J.J., et al. (1991). Anal. Biochem. 194, 156-162.
- Greenfield, R.S., Kaneko, T., Daues, A., Edson, M.A., Fitzgerald, A., Olech, L.J., Grattan, J.A., Spitalny, G.L. and Braslawsky, G.R. (1990). Evaluation in vitro of adriamycin immunoconjugates synthesized using an acid sensitive hydrazone linker. Cancer Res. 50, 6600-6607.
|
