An amine-to-sulfhydryl crosslinker with a short spacer arm (7.3 angstroms).
Thermo Scientific Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).
Low potential for eliciting an immune response, ensuring that the
primary response is to the antigen-carrier protein conjugate and not
against a determinant on the crosslinker
Less immunogenic than SMCC
Reactive groups: NHS ester and maleimide
Reactive toward: amino and sulfhydryl groups
Sulfonated, water-soluble analog also available; see Sulfo-GMBS.
Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15), 3031-3039.
Fujiwara, K., et al. (1988). Sandwich enzyme immunoassay of tumor-associated antigen sialosylated Lewis (x) using ß-D-galactosidase coupled to a monoclonal antibody of IgM isotype. J. Immunol. Meth. 112, 77-83.
Formulation: N-gamma-Maleimidobutyryl-oxysuccinimide ester; powder solid Sufficient For: 178mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use