Thermo Scientific Pierce KMUH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
- Sulfhydryl-reactive and carbonyl-reactive heterobifunctional crosslinking agent
- Long, non-cleavable, aliphatic cross-bridge
- Hydrazide group covalently couples to oxidized carbohydrate residues in glycoproteins and other glycoconjugates
- Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
- Reactive groups: maleimide and hydrazide
- Reactive toward: sulfhydryl and carbonyl (aldehyde)/carboxyl groups
A guide to crosslinking and protein modification
An overview of glycosylation and methods to analyze glycoproteins and glycopeptides
- Trail, P.A., et al. (1993). Cure of xenografted human carcinomas by BR96-doxorubicin immunoconjugates. Science 261, 212-215.
See also: BMPH, EMCH, MPBH, PDPH.