EMCS

Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).

Highlights:

• NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable, water-insoluble
• Increased sphere of coupling vs. GMBS
• Aliphatic spacer offers low potential for eliciting an immune response
• Reactive groups: maleimide and NHS ester
• Reactive toward: sulfhydryl and amino groups
• Sulfonated, water-soluble analog also available; Sulfo-EMCS.  See also: AMAS, BMPS, GMBS.

Product Details:

References:

1. Fujiwara, K., et al. (1988). J. Immunol. Methods 112, 77-83.
2. Peeters, J.M., et al. (1989). J. Immunol. Methods 120, 133-143.
3. Hermanson, G.T. (1996). Bioconjugate Techniques, San Diego: Academic Press, pp. 243-245. (EMCS use can be modeled after GMBS.)