BMPS

Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 angstroms).

Highlights:

• Reactive groups: maleimide and NHS ester
• Reactive toward: sulfhydryl and amine groups
• C₃ homolog of aliphatic spacer series (AMAS, GMBS and EMCS) with identical reactivities
• NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
• Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable and water-insoluble
• Aliphatic spacer has low potential for eliciting an immune response, ensuring that the primary response to the antigen-carrier protein is not diluted by a response against a determinant on the crosslinker

Product Details:

References:

1. McKenzie, J.A., Raison, R.L. and Rivett, E.E. (1988). Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins. J. Protein Chem. 7(5), 581-592.
2. Hermanson, G.T. (1996). Bioconjugate Techniques. San Diego: Academic Press (pp. 266-272). (Product # 20002T)
3. 1. Kitagawa, T., et al. (1981). Chem. Pharm. Bull. 29(4), 1130-1135.
4. Kitagawa, T. (1981). "Enzyme labeling with N-hydroxysuccinimidyl ester of maleimide", In Ishikawa, E., Kawai, T. and Miyai, K. (Eds.), Enzyme Immuno-assay (pp. 81-89). IGAKU-SHOIN, Tokyo-New York.