An amine-to-sulfhydryl crosslinker with a short spacer arm (5.9 angstroms).
Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 angstroms).
Reactive groups: maleimide and NHS ester
Reactive toward: sulfhydryl and amine groups
C3 homolog of aliphatic spacer series (AMAS, GMBS and EMCS) with identical reactivities
NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
Non-cleavable and water-insoluble
Aliphatic spacer has low potential for eliciting an immune response, ensuring that the primary response to the antigen-carrier protein is not diluted by a response against a determinant on the crosslinker
McKenzie, J.A., Raison, R.L. and Rivett, E.E. (1988). Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins. J. Protein Chem.7(5), 581-592.