Thermo Scientific Pierce BMPH is a short, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
- Reactive groups: maleimide and hydrazide
- Reactive toward: sulfhydryl groups and carbonyl (aldehyde)/carboxyl groups
- Sulfhydryl-reactive and carbonyl-reactive heterobifunctional reagent
- Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
- Water-soluble and non-cleavable
- Useful for covalently coupling to oxidized carbohydrate moieties in glycoproteins and other glycoconjugates
A guide to crosslinking and protein modification
An overview of glycosylation and methods to analyze glycoproteins and glycopeptides
- Chrisey, L.A., Lee, G.U. and O’Ferrall, C.E. (1996). Covalent attachment of synthetic DNA to self-assembled monolayer films. Nucleic Acids Research 24(15), 3031-3039.
- Kitagawa, T., Shimozono, T., Aikawa, T., Yoshida, T. and Nishimura, H. (1980). Preparation and characterization of hetero-bifunctional crosslinking reagents for protein modifications. Chem. Pharm. Bull. 29(4), 1130-1135