An amine-to-sulfhydryl crosslinker with a very short spacer arm (4.4 angstroms).
Thermo Scientific Pierce AMAS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a very short spacer arm (4.4 angstroms).
Non-cleavable, close proximity crosslinker
Aliphatic spacer has low potential for eliciting an immune response
NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
Functional homolog of BMPS, GMBS and EMCS
Reactive groups: maleimide and NHS ester
Reactive toward: sulfhydryl and amino groups
May, J.M. (1989). Selective labeling of the erythrocyte hexose carrier with a maleimide derivative of glucosamine: relationship of an exofacial sulfhydryl to carrier conformation and structure. Biochemistry28, 1718-1725.
Sayre, L.M., Larson, D.L., Takemori, A.E. and Portoghese, P.S. (1984). Design and synthesis of naltrexone-derived affinity labels with nonequilibrium opioid agonist and antagonist activities. Evidence for the existence of different mu receptor subtypes in different tissues. J. Med. Chem.27(10), 1325-1335.
Formulation: N-alpha-Maleimidoacet-oxysuccinimide ester; solid Sufficient For: 198mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use