AMAS N-(a-Maleimidoacetoxy) succinimide ester)

Thermo Scientific Pierce AMAS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a very short spacer arm (4.4 angstroms).

Highlights:

• Non-cleavable, close proximity crosslinker
• Aliphatic spacer has low potential for eliciting an immune response
• NHS ester end couples with primary amines at pH 7-9, forming stable amide bonds
• Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Functional homolog of BMPS, GMBS and EMCS
• Water-insoluble
• Reactive groups: maleimide and NHS ester
• Reactive toward: sulfhydryl and amino groups

Product Details:

References:

1. May, J.M. (1989). Selective labeling of the erythrocyte hexose carrier with a maleimide derivative of glucosamine: relationship of an exofacial sulfhydryl to carrier conformation and structure. Biochemistry 28, 1718-1725.
2. Sayre, L.M., Larson, D.L., Takemori, A.E. and Portoghese, P.S. (1984). Design and synthesis of naltrexone-derived affinity labels with nonequilibrium opioid agonist and antagonist activities. Evidence for the existence of different mu receptor subtypes in different tissues. J. Med. Chem. 27(10), 1325-1335.