DTME

Thermo Scientific Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide cysteines) by reduction of the disulfide bond in the center of the spacer arm.

Highlights:

• Cleavable by reducing agents
• Reactive groups: maleimide (homobifunctional)
• Reactive toward: sulfhydryl groups
• Intermediate length, sulfhydryl-reactive crosslinker
• Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Cleavable by DTT, 2-mercaptoethanol or TCEP
• Water-insoluble

Product Details:

References:

1. Chen, L.L., Rosa, J.J., Turner, S. and Pepinsky, R.B. (1991). Production of multimeric forms of CD4 through a sugar-based crosslinking strategh. J. Biol. Chem. 266(27), 18237-18243.
   
2. Han, J.C. and Han, G.Y. (1994). A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothretiol. Anal. Biochem. 220, 5-10.