A mid-length, maleimide crosslinker with an internal disulfide bond for reversible sulfhydryl conjugation.
Thermo Scientific Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide cysteines) by reduction of the disulfide bond in the center of the spacer arm.
Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
Cleavable by DTT, 2-mercaptoethanol or TCEP
Chen, L.L., Rosa, J.J., Turner, S. and Pepinsky, R.B. (1991). Production of multimeric forms of CD4 through a sugar-based crosslinking strategh. J. Biol. Chem.266(27), 18237-18243.
Han, J.C. and Han, G.Y. (1994). A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothretiol. Anal. Biochem.220, 5-10.
Formulation: Dithiobismaleimidoethane; solid Sufficient For: 800mL of typical 1X (0.2mM) reaction solution, or approx. 25 uses at 2mg reagent per use