A base-cleavable reagent for reversible crosslinking between amines.
Thermo Scientific Pierce BSOCOES is a homobifunctional NHS-ester crosslinker with a base-cleavable spacer arm for reversible crosslinking between primary amines.
Base cleavable (pH 11.6, 2 hours, 37°C)
Reactive groups: NHS ester (homobifunctional)
Reactive towards: amino groups
Bouizar, Z., Fouchereau-Person, M., Taboulet, J., Moukhtar, M.S. and Milhaud, G. (1986). Purification and characterization of calcitonin receptors in rat kidney membranes by covalent crosslinking techniques. Eur. J. Biochem.155, 141-147.
Zarling, D.A., Watson, A. and Bach, F.H. (1980). Mapping of lymphocyte surface polypeptide antigens by chemical crosslinking with BSOCOES. J. Immunol.124, 913-920.
Formulation: Bis[2-(succinimidooxycarbonyloxy)ethyl]sulfone; powder solid Sufficient For: 115mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use