The accuracy of the Lowry method in a ready-to-use formulation.
The Thermo Scientific Pierce Modified Lowry Protein Assay is a stable form of a traditional, 2-component, folin phenol- and copper-based reagent system to measure (A750nm) total protein concentration compared to a protein standard.
The Modified Lowry Protein Assay Kit combines a stabilized formulation of the original Lowry Reagents and the essential Folin-Ciocalteu Phenol reagent in a complete kit for accurately determining protein concentration in a variety of samples types. Although newer protein assay methods provide greater speed and convenience, the Lowry method remains a popular, accurate and useful option for many applications.
Popular method – widely cited in protein research literature
Colorimetric – measure with a standard spectrophotometer or plate reader (750nm)
Stable – uses a modified cupric sulfate-tartrate reagent that is stable at room temperature
Large range – exhibits good linearity in the range 1 to 1500µg/mL (tested with BSA protein)
Convenient – microplate and cuvette protocols provided with the instructions
Protocol summary for the Thermo Scientific Modified Lowry Protein Assay.
Standard curves. Typical standard curves for bovine serum albumin (BSA) and bovine gamma globulin (BGG) in the Modified Lowry Protein Assay. Kit includes ampules of Albumin Standard.
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Nagai, Y., et al. (2000). J. Biol. Chem. 25, 10437-10442.