Micro BCA Protein Assay Kit

The Thermo Scientific Pierce Micro BCA Protein Assay is a special 3-component version of our popular BCA Reagents, optimized to measure (A562nm) total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA Reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.

Highlights:

• Sensitive – accurately detect down to 0.5µg/mL (2µg/mL in microplate format)
• High linearity – linear working range for BSA equals 0.5 to 20µg/mL
• Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
• Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
• Convenient – microplate and cuvette protocols provided with the instructions
• Stable – kit stable at room temperature; prepared working reagent stable for to 24 hours

Product Details:

Protocol summary for the Thermo Scientific Pierce Micro BCA Protein Assay .

Standard curves. Typical standard curves for bovine serum albumin (BSA) and bovine gamma globulin (BGG) in the Micro BCA Protein Assay Kit. The kit includes ampules of Albumin Standard.

References:

1. Smith, P.K., et al. (1985). Anal. Biochem. 150(1), 76-85.
2. Kang, D.E., et al. (2002). Cell 110, 751-762.
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4. Blum, D., et al. (2002). J. Neurosci. 22, 9122-9133.
5. Paratcha, G., et al. (2003). Cell 113, 867-879.