Specially formulated for measuring dilute protein solutions.
The Thermo Scientific Pierce Micro BCA Protein Assay is a special 3-component version of our popular BCA Reagents, optimized to measure (A562nm) total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA Reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.
Sensitive – accurately detect down to 0.5µg/mL (2µg/mL in microplate format)
High linearity – linear working range for BSA equals 0.5 to 20µg/mL
Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
Convenient – microplate and cuvette protocols provided with the instructions
Stable – kit stable at room temperature; prepared working reagent stable for to 24 hours
Protocol summary for the Thermo Scientific Pierce Micro BCA Protein Assay .
Standard curves. Typical standard curves for bovine serum albumin (BSA) and bovine gamma globulin (BGG) in the Micro BCA Protein Assay Kit. The kit includes ampules of Albumin Standard.
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