Fluoraldehyde Reagent

Thermo Scientific Pierce Fluoraldehyde (OPA) Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Reagent contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation. The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations. The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution.

Highlights:

• For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
• Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives
• Provides an accurate measure of both composition and absolute protein-peptide content
• Ideal for work with recombinant proteins and synthetic peptides
• Can be used for fluorescent protein or peptide assay
• Pre-column derivatization mixtures can be injected into LC without any processing

Reagent Properties:

• Formulation: 0.8mg/mL o-phthalaldehyde (OPA) in proprietary buffer, pH 10
• Form: Clear, colorless to slightly yellow liquid
• Reactive toward: Primary amines (–NH2)
• Detection: Fluorescence (excitation/emission = 340nm/455nm)
• Application: Pre- or post-column derivatization for detection by HPLC

Product Details:

Chemical structure of ortho-phthalaldehyde (OPA).Thermo Scientific Fluoraldehyde Reagent is a ready-to-use solution of 0.8mg/mL o-phthalaldehyde (OPA) in proprietary buffer, pH 10. Crystalline OPA is also available (Part No. 26015).

Phthaldehyde (OPA) derivatization reaction. The rapid OPA-amine reaction in the presence of 2-mercaptoethanol yields an intense blue fluorescent product that has maximum excitation and emission wavelengths of 340 and 455nm, respectively. Thermo Scientific Fluoraldehyde Reagent is a ready-to-use solution that contains OPA and 2-ME.

Excellent linearity and high sensitivity with ortho-phthalaldehyde (OPA) derivatization. Experiment with Thermo Scientific Fluoraldehyde Reagent shows excellent linear response, whether in the 2500 or 200 picomole range.

Outstanding shelf life. Comparison of fluorescence response of selected amino acids after reaction with new and one-year-old Fluoraldehyde Reagent Solutions. Peak height and quality are nearly as good with the older solution.

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