Fluoraldehyde o-Phthalaldehyde Crystals

Thermo Scientific Pierce Fluoraldehyde Crystals are o-phthalaldehyde (OPA), a highly sensitive fluorescent derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Crystals are a specially purified, fluorogenic-grade preparation of o-phthalaldehyde (OPA). This amine-reactive compound is primarily used for high-sensitivity, fluorescent detection of primary amines, including amino acids, peptides, proteins and polyamines in pre- or post-column chromatographic effluents.

Highlights:

• For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
• Reacts with all primary amine-containing analytes to yield fluorescent derivatives (ex/em = 340nm/455nm)
• Provides an accurate measure of both composition and absolute protein-peptide content
• Ideal for work with recombinant proteins and synthetic peptides
• Can be used for fluorescent protein or peptide assay
• Can be prepared as a stable aqueous solution in borate (see instructions)

Reagent Properties:

• Alternative names: Fluoraldehyde Crystals, OPA
• Chemical name: ortho-Phthalaldehyde
• Reactive toward: Primary amines (–NH2)
• Chemical formula: C8H6O2
• CAS number: 643-79-8
• Molecular weight: 134.12
• Form: Pale yellow, free-flowing crystals

Product Details:

Chemical structure of ortho-phthalaldehyde (OPA). Thermo Scientific Fluoraldehyde Crystals are high-purity crystalline OPA. For HPLC applications, OPA is usually used as reagent solution containing 2-mercaptoethanol. Consider using our ready-to-use Fluoraldehyde Reagent (Part No. 26025).

For even greater sensitivity, use a combination of OPA and Fmoc-Chloride with automated pre-column derivatization to detect both primary and secondary amines. With this application, primary amino acids are first derivatized with OPA and then remaining secondary amino acids are reacted with Fmoc-Chloride, resulting in extraordinary amino acid detection sensitivity and accuracy (see Lindroth and Mopper, 1979; Lee and Drescher, 1979).

References:

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