 Thermo Scientific PEPotec SRM Peptide Libraries provide fully synthetic, crude peptides customized for the development of mid- to high-throughput selected reaction monitoring (SRM) and multiple reaction monitoring (MRM) assays in quantitative mass spectrometry (MS) workflows.
The study of proteomes, sub-proteomes and protein pathways often requires quantitative MS analysis that depends on the identification and validation of SRM and MRM assays. PEPotec SRM peptide libraries offer great convenience and flexibility in the development of quantitative MS approaches while accelerating biological assays by reducing the setup time of MS experiments. These peptide libraries were developed as a result of our involvement in the SRMAtlas project, which seeks to map the entire human proteome.
The standard service supplies peptides with either arginine (R) or lysine (K) as the C-terminal amino acid, which encompass the most commonly used tryptic proteotypic peptides used for SRM assay development. While the standard maximum peptide length is 25 amino acids, this length can be optionally increased to 35 amino acids to ensure that the vast majority of potential proteotypic peptides that are screened for their suitability for a reliable SRM assay are covered.
Highlights:
- Convenient – peptides are provided in individual 2-D barcoded tubes in 96-tube plates
- Application-specific – C-terminal amino acid of each peptide can be either R or K
- Easy to use – peptides can be delivered lyophilized or suspended in 0.1% trifluoroacetic acid (TFA) in 50% (v/v) acetonitrile/water
- Flexible – extensive list of available modifications
Includes:
- Fully synthetic crude (as synthesized) peptides
- Standard mass spectrometric quality control (QC) analysis of 5% of samples plus one control peptide
- Optional modifications, peptide sizes and levels of QC analysis
- Provided in individual Thermo Scientific Matrix 96-tube plates
Applications:
- Mid- to high-throughput development of SRM and MRM assays
- MS workflows with relative and absolute quantitation strategies
| PEPotec SRM Peptide Libraries, Standard Service |
| Quantity |
>0.1mg |
| Length |
6 to 25 amino acids; L-isoforms only |
| Purity |
Crude (as synthesized) |
| QC |
MS, 5% of samples plus 1 control peptide |
| Formulation |
Suspended in 0.1% TFA in 50% (v/v) acetonitrile/water
or lyophilized |
| Vessel |
Thermo Scientific Matrix 96-tube plates (Part No. 3712MTX) |
| Minimum Order |
24 peptides |
| C-terminal amino acid restrictions |
R or K |
| Counter-ion |
TFA |
| PEPotec SRM Peptide Sequence, Length and Order Options |
| Part No. |
Modification/Service |
Unit Size |
| 500507 |
Choice of C-terminal amino acid (excluding cysteine) |
Peptide |
| 500553 |
Peptides that are 3-5 or 26-35 amino acids in length |
| 500529 |
Orders with <24 peptides |
Order |
| PEPotec SRM Peptide Optional Modifications |
| Part No. |
Service |
Unit Size |
| 500503 |
Phosphorylation at 1 site |
Peptide |
| 500504 |
Phosphorylation at 2 sites |
| 500549 |
Phosphorylation at 3 sites |
| 500505 |
All cysteines protected by carbamidomethylation (CAM) |
| 500515 |
Lysine acetylation at 1 site |
| 500011 |
Methionine sulfoxide |
| 500520 |
Methyl lysine at 1 site |
| 500522 |
Methyl lysine |
| 500527 |
Diglycine ubiquitination motif on lysine |
| 500528 |
3-chlorotyrosine |
| 500535 |
Methyl arginine |
| 500540 |
Isoaspartic acid |
| 500544 |
Formylation |
| 500548 |
Hydroxyproline |
| 500550 |
Isotopic labeling at C-terminus with heavy R or K |
| 500551 |
Isotopic labeling at C-terminus with heavy A, I, L, F, P or V |
| 500508 |
Isotopic labeling at single internal site with heavy amino acid |
| Please email us to find out other modifications that may be available for your specific order. |
| PEPotec SRM Peptide Library Optional QC Services |
| Part No. |
Service |
Unit Size |
| 500512 |
QC: MS of 100% of samples; includes MS certificate and 1 resynthesis if correct peptide peak not found during analysis |
Order |
| 500513 |
QC: MALDI MS + analytical HPCL of 100% of samples; includes documentation |
| 500552 |
QC: MS per peptide on orders with <24 peptides |
References:
- ISB, ETH Zurich Complete Initial Draft of SRMAtlas Covering Entire Human Proteome. Bonislawski, A. GenomeWeb.com. Sept. 24, 2010.
Related Resources:
Peptide Analysis Tool
Antigen Profiler for Robust Peptide Antibody Design
A review of peptide design
A review of peptide synthesis
Related Products:
PEPotec Immuno Custom Peptide Libraries
Custom Peptide Synthesis Service
HeavyPeptide AQUA Custom Synthesis Service
HeavyPeptide AQUA FasTrack Service
HeavyPeptide IGNIS Prime Peptide Quantitation Kit
Peptide Sample Prep Tools for Mass Spectrometry
Amino Acid Analysis Reagents and Accessories
Quote Request and Ordering Information |
To obtain a quote:
- Download the Quote Request/Order Form (1.7mb)
- Complete all contact and billing information
- List peptides, modifications and optional services
|
For North America
Email quote requests or orders to
peps@thermofisher.com
OR
Contact your local sales representative or call 1-800-874-3723 or 1-815-968-0747. |
For Europe and all other regions
Email quote requests to services.biopolymers@thermofisher.com
Email orders to sales.peptides@thermo.com
Or contact your local sales representative or Thermo Fisher Scientific in Ulm, Germany at +49-731-29870. |
For technical support, please call
1-800-874-3723 or 1-815-968-0747 |
For technical support, please call +49-731-29870. |
|
