Thermo Scientific Pierce Stabilized HRP Conjugates are standard peroxidase-labeled anti-mouse IgG and anti-rabbit IgG secondary antibodies that are pre-diluted (10µg/mL) for convenient pipetting with immunodetection methods.
These dilute but stable antibody stock solutions provide for greater accuracy and convenience in preparing probing solutions for ELISA, Western blotting and other detection procedures. The conjugates are accurately prepared, dispensed and supplied at 10µg/mL (100X more dilute than typical 1mg/mL stocks). This eliminates inaccuracies and pipetting labor associated with two-stage dilution schemes required with traditional conjugate preparations for use with chemiluminescent substrates and other high-sensitivity detection methods. The liquid formulation of each prediluted HRP-conjugate is stable at 4°C, eliminating the need to freeze stock solutions for storage.
- Obtain high signal-to-noise ratio – ideal for Western Blotting, ELISA and IHC applications
- Get reproducible results – avoid inconsistencies caused by lot-to-lot variation and magnification of pipetting error during dilution
- Save time – stabilized liquid formats eliminate the need to freeze aliquots; no waiting time for thawing aliquots
- Ready to use – format of the stabilized liquid conjugates provides ease of use and handling
||Superb Western blot results with Thermo Scientific Pierce Stabilized HRP Conjugates. Western blot of HeLa lysate samples probed with a mouse anti-GAPDH antibody (1:2000 dilution of 1mg/mL stock solution) and then Stabilized Goat Anti-Mouse-HRP (4ng/mL, 1:2500 dilution of 10µg/mL stock solution). HRP activity was detected with SuperSignal West Femto Chemiluminescent Substrate (Part No. 34095). If a typical 1mg/mL stock solution of secondary antibody had been used, it would have required a 250,000-fold dilution, which is both tedious, prone to pipetting error and usually wasteful.
These goat anti-mouse IgG whole molecule (GAM) and goat anti-rabbit IgG whole molecule (GAR) are conjugated to horseradish peroxidase using an optimized manufacturing procedure that ensures complete conjugation of the proteins to HRP. This is a key feature, as any unconjugated protein would compete with the conjugated material and cause lower signal intensity in downstream applications. We have used our HRP conjugates in ELISA, Western blotting and immunohistochemistry (IHC) applications, demonstrating their competence in these applications.
All Secondary Antibodies and Conjugates
Standard Anti-Mouse IgG Antibodies
Standard Anti-Rabbit IgG Antibodies
Pierce ECL and SuperSignal Chemiluminescent Western Blotting Substrates