Thermo Scientific Pierce Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that are popular blocking agents for immunohistochemical (IHC) staining and cellular imaging methods.
Normal serum that is derived from the same or related species of animal as a tissue sample or from the host species of specific antibodies used in immunodetection method provides several properties that improve assay performance for immunohistochemistry. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components.
- Multiple species available – choose normal serum from goat, horse, human, mouse, rabbit, rat and swine
- Trouble-free formulation – delipidated (lipid-extracted) and dialyzed against weak phosphate-buffered saline (PBS) containing sodium azide
- Stable format – supplied lyophilized (from PBS with preservative) for long shelf life and easy reconstitution in water
- Safe source – USA is country of origin for all these animal-derived products
Normal serum is the term used to describe serum from an animal that has not been immunized to produce antibodies against specific antigens. Thus, normal serum is the opposite of anti-serum. Normal sera contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods.
- Alegria-Schaffer, A., et al. (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.
Protein Methods Library: Blocking Buffers for Western Blotting and ELISA
Protein Methods Library: Overview of Immunohistochemistry
All Blocking Buffers
Nonspecific Antibodies (purified immunoglobulins and gamma globulins)