Blocking Buffers for Protein Methods

Traditional protein blocking agents (BSA, casein and milk) and exclusive Pierce blocking buffers (SuperBlock, StartingBlock, Protein-Free) for Western blotting, ELISA and other immunoassay detection methods with microplates and membranes.

Selection Guides – Choose a blocking buffer for your experiment:
- Blocking Buffers Selection Guide
Protein Methods Library – Learn more about blocking buffers:
- Blocking Buffers for Western Blotting and ELISA
- Blocking Strategies for Immunohistochemistry (IHC)
Related Product Categories – Other products of interest:
- PBS, TBS and other Buffers for Protein Methods


	Blocker BLOTTO Blocking Buffer Ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA, IHC and other detection methods.


	Blocker BSA Blocking Buffers Ready-to-use 10X PBS or TBS solutions of bovine serum albumin for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.


	Blocker Casein Blocking Buffers Ready-to-use PBS or TBS solutions of purified casein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.


	Pierce Clear Milk Blocking Buffer A long shelf-life replacement for homemade milk blocking buffers based on non-fat dry milk, designed especially for Western blotting applications.


	Pierce Fast Blocking Buffer Block and wash Western blots to achieve comparable results in much less time using this 5-minute blocker solution and rapid wash buffer for nitrocellulose or PVDF.


	Pierce Normal Serum Non-immune serum samples from goat, human, mouse, rabbit and other species that are popular blocking agents for IHC and cellular imaging.


	Pierce Protein-Free Blocking Buffers PBS and TBS formulations of a non-protein compound for effective membrane and plate blocking with extremely low background.


	SEA BLOCK Blocking Buffer Steelhead salmon serum in PBS especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.


	StartingBlock Blocking Buffers PBS and TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility with membrane- and plate-based detection.


	SuperBlock Blocking Buffers Optimized PBS and TBS solutions containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.