Secondary Antibody Coated Plates

Thermo Scientific Pierce Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates.

Because these plates are IgG-specific, purified antibodies are not required. Pierce Antibody Coated Plates may be used for direct, indirect, competitive or sandwich assays and are available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.

Highlights:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Anti-mouse plates: Binding Capacity: ~7pmol IgG/well
• Anti-rabbit plates: Binding Capacity: ~12pmol IgG/well
• Activation level: 100µL/well

References:

1. Gartner, W., et al. (2001). Cereb Cortex. 11, 1161-1169.
2. Wagner, L., et al. (2000). J. Biol. Chem. 275, 24740-24751.