Pre-blocked and ready to capture biotinylated antibodies and other molecules.
Thermo Scientific NeutrAvidin Protein Coated Plates are pre-blocked and ready-to-use polystyrene 96-well and 384-well plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.
These Thermo Scientific Pierce Coated Plates are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.
Highlights:
Easy and gentle immobilization of biotin-containing conjugates
Low nonspecific binding
No denaturing of the protein component of a conjugate upon binding
Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer
Less expensive than streptavidin coated plates
Lowest nonspecific binding properties of all biotin-binding proteins
Product Details:
Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.
Plate Characteristics for Standard Capacity Biotin-Binding Plates. The coating and blocking volumes listed here are the most common varieties. Individual Part Nos. may be different.
