Pre-blocked and ready to capture biotinylated antibodies and other molecules.
Thermo Scientific NeutrAvidin Protein Coated Plates are pre-blocked and ready-to-use polystyrene 96-well and 384-well plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.
These Thermo Scientific Pierce Coated Plates are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.
Easy and gentle immobilization of biotin-containing conjugates
Low nonspecific binding
No denaturing of the protein component of a conjugate upon binding
Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer
Less expensive than streptavidin coated plates
Lowest nonspecific binding properties of all biotin-binding proteins
Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.
Plate Characteristics for Standard Capacity Biotin-Binding Plates. The coating and blocking volumes listed here are the most common varieties. Individual Part Nos. may be different.