Highly sensitive immunohistochemistry detection for biotinylated antibodies.
Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.
Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Highly diluted primary antibodies can be used with Pierce Staining Kits, producing comparable stain intensity to other methods that require higher concentrations of antibody. A Standard Kit includes only the avidin and biotinylated enzyme and is useful if an ABC Kit is not available for your specific species of primary antibody or if the specificity of the antibody in the kit is not appropriate for the application. The biotinylated antibody for your specific application can then be purchased separately, along with blocking serum, and used with a standard kit. The Ultra-Sensitive ABC Peroxidase Staining Kits are more sensitive than the ABC Peroxidase Staining Kits without exhibiting increased background staining. These kits supply the extra sensitivity needed for localizing antigens present in very small quantities. An expensive primary antibody may be diluted approximately five-fold higher than with the ABC Peroxidase Kit while producing equal staining intensity.
Pierce ABC Kit Contents:
3 mL of the appropriate blocking serum
1 mL of the biotinylated affinity-purified secondary antibody
2 mL each of the avidin and biotinylated enzyme reagents
References:
Savage,M.D., Mattson,G.Desai,S.,Nielander,G.W.,Morgensen,S.and Conklin,E.J.(1992). Avidin-Biotin Chemistry:A Handbook.Rockford,Illinois:Pierce Chemical Company
Bayer,E.A,Ben-Hur,H.,Wilcheck,M.(1988).Biocytin-hydrazide:a selective label for sialic acids,galactose, and other sugars in glycoconjugates using avidin/biotin technology,Anal.Biochem. 170,271-281
