Thermo Scientific EZ-Link Psoralen-PEG3-Biotin enables biotinylation of DNA or RNA via UV-light-activated intercalation of the psoralen group with thymine- and other pyrimidine-containing bases to form a covalent bonds.
Psoralen-PEG3-Biotin is a photoactivatable reagent for biotinylation of DNA or RNA. Upon activation with UV-Light (~350nm), the psoralen group reacts with thymine- and other pyrimidine-containing bases to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.
Highlights:
- Biomolecular labeling – biotinylate DNA or RNA (approximately 1 biotin per 10 to 20 bases) without interfering with hybridization
- Photo-reactive – covalent attachment occurs in the presence of UV light (~350nm) for 10 to 30 minutes
- Psoralen-activated – reacts through a cyclo-addition mechanism with the 5,6 double bond in thymine- and other pyrimidine-containing bases
- Pegylated – spacer arm contains a hydrophilic, 3-unit, polyethylene glycol (PEG) group
- Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
- Long reach – spacer arm (total length added to target) is 36.9 angstroms, minimizing steric hindrance for binding interactions with streptavidin
Product Details:
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| Chemical structure of Psoralen-PEG3-Biotin. This biotinylation reagent covalently attaches to nucleic acids upon photo-activation with UV-light. For more information, see our review of Photoreactive Conjugation Chemistry. |
| Properties of Psoralen-PEG3-Biotin. This photo-activatable compound has an extended, pegylated spacer arm terminated by a psoralen reactive group. |
SMILES structure:
O=C1OC2=C(OCCCC(NCCOCCOCCOCCNC(CCCCC(SC3)C4C3NC(N4)=O)=O)=O)C5=C(C=CO5)C=C2C=C1 |
| Molecular formula |
C33H44N4O10S |
| Molecular weight |
688.79 |
| Spacer arm length |
36.9 angstroms |
| Form |
White to off-white solid |
| Storage conditions |
4°C protected from light |
| Reactive groups |
Psoralen, reacts upon exposure to ultraviolet light |
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Applications:
- Selective labeling of cell surface proteins (Ref.1)
- Biotinylation of hyaluronan (Ref.2)
- Labeling of endotoxin for receptor binding studies (Ref.3)
References:
- Henriksen, U., et al. (1991). J. Photochem. Photobiol. A: Chem. 57, 331-342.
- Oser, A., et al. (1988). Nucleic Acids Res. 16, 1181-1196.
- Wassarman, D.A. (1993). Mol. Biol. Rep. 17, 143-151.
- Cimono, G.D., et al. (1985). Annu. Rev. Biochem. 54, 1151-1193.
- Wassarman, D.A. (1993). Mol. Biol. Rep. 17, 143-151.
Related Resources:
Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)
Related Products:
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins
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