Thermo Scientific EZ-Link ASA-LC-Biotin is an iodinatable and photoactivatable reagent that enables biotinylation of nucleic acids and other molecules that do not have readily available amine or sulfhydryl groups for coupling.
ASA-LC-Biotin is an iodinatable and photoactivatable reagent that enables biotinylation of nucleic acids and other molecules that do not have readily available amine or sulfhydryl groups for coupling. When exposed to strong ultraviolet or visible light, the aryl azide group of ASA-LC-Biotin converts to a reactive nitrene that readily reacts to form covalent bonds with a variety of chemical groups. When photoactivation occurs in the presence of single- or double-stranded DNA or RNA, the nucleic acid becomes covalently labeled with biotin. This process is quick and simple, eliminating the problems encountered with nick translation.
- Nucleic acid labeling – biotinylate DNA, RNA and other macromolecules that do not possess primary amines or sulfhydryl groups
- Photo-reactive – azido group activates upon exposure to ultraviolet light to form covalent bonds with nucleophiles and many other chemical groups
- Iodinatable – tyrosyl-like ring can be labeled with iodine (125I) to make radioactive probes
- Irreversible – forms permanent thioether bonds; spacer arm cannot be cleaved
- Solubility – best to dissolve in DMSO or DMF before further dilution in aqueous buffers
- Medium length – spacer arm (total length added to target) is 29.9 angstroms
|Chemical structure of ASA-LC-Biotin. This biotinylation reagent covalently attaches to proteins and other molecules upon photo-activation with UV-light. For more information, see our review of Aryl Azide Reaction Chemistry. If the application does not require iodination, consider using TFPA-PEG3-Biotin instead of ASA-LC-Biotin.
|Properties of ASA-LC-Biotin. This photo-activatable compound has an extended spacer arm terminated by a hydroxyphenyl azide reactive group.
|Spacer arm length
|Mass added to target
||Several products possible
||White to cream colored powder
||4°C protected from light
||Phenyl azide, reacts upon exposure to ultraviolet light
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Biotinylation reagents differ in reactivity, length, solubility, cell permeability and cleavability. Several different types of photoreactive compounds are available. Aryl azide reagents activate upon exposure to ultraviolet light initiate addition reactions with double bonds, insertion into C–H and N–H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines).
Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins