Thermo Scientific Pierce Avidin and Conjugates include purified avidin protein from hen egg whites, plus avidin-HRP, avidin-AP and other conjugated forms for use in biotin-based detection methods.
Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.
- Native avidin – glycoprotein from chicken egg whites, 67kDa, pI = 10
- Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin
- Affordable – significantly less expensive than recombinant streptavidin
- ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
- Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays
Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification.
- Gitlin, G., Bayer, E.A. and Wilchek, M. (1987). Biochem. J. 242, 923-926.
- Bruch, R.C. and White, H.B., III (1982). Biochemistry 21, 5334-5341.
- Green, N.M. (1975). Avidin. Adv. in Protein Chemistry. New York: Academic Press, pp. 29, 85-133.
- Montgomery, R. (1972). Glycoproteins. BBA Library, Vol. 5A, Gottschalk, A., ed. Amsterdam: Elsevier, p. 519.
- Zuk, P.A. and Elferink, L.A. (2000) J. Biol. Chem. 275, 26754-26764.
Protein Methods Library: Immunodetection strategies for IHC
All Biotin-Binding Proteins
Agarose Resins of Avidin and Streptavidin
ABC Staining Kits
HABA Dye for biotin quantitation