Thermo Scientific Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
This purified horseradish peroxidase (HRP) is supplied lyophilized as a salt-free powder for reconstitution and use in protein research methods. The main application for HRP in molecular biology and protein research is as a reporter system for immunoassays and other probe-based assay techniques such as ELISA, Western blotting, EMSA and Southern blotting. The enzyme is usually conjugated to specific secondary antibodies or streptavidin, and its activity is detected with a color-forming (or light-generating) substrate.
- Purified form – lyophilized salt-free powder; ready to dissolve and use
- High specific activity – typically greater than 300 units/mg (lot-specific value reported)
- Compared to AP – HRP is smaller (40kDa) than alkaline phosphatase (AP; 140kDa) and has higher specific enzyme activity than both AP and beta-galactosidase (b-Gal)
- Many options – numerous substrate solutions and assay techniques are available for HRP
One unit catalyses the production of 1mg of purpurogallin from pyrogallol in 20 seconds at 20°C and pH 6.0.
- Porstmann, B., Porstmann, T., Nugel, E. and Evers, U. (1985). Which of the commonly used marker enzymes gives the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline phosphatase, ß-galactosidase? J. Immunol. Meth. 79, 27-37.
- Wordinger, R.J., Miller, G.W. and Nicodemus, D.S. (1987). Manual of Immunoperoxidase Techniques, 2nd Edition. Chicago: American Society of Clinical Pathologists Press, pp. 23-24.
- Yolken, R.H. (1982). Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects. Rev. Infect. Dis. 4(1), 35-68.
- Cordell, J.L., et al. (1984). Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem. 32, 219-229.
- Passey, R.B., et al. (1977). Evaluation and comparison of 10 glucose methods and the reference method recommended in the proposed product class standard. Clin. Chem. 23(1), 131.
- Hosoda, H., Takasaki, W., Tsukamoto, R. and Nambara, T. (1987). Sensitivity of steroid immunoassays. Comparison of alkaline phosphatase, ß-galactosidase and horseradish peroxidase as labels in a colorimetric assay system. Chem. Pharm. Bull. 35, 3336-3342.
- Samoszuk, M.K., et al. (1989). Antibody, Immunoconjugates and Radiopharmaceuticals 2, 37-46.
Review of Immunohistochemistry (IHC) vs. Immunocytochemistry
Review of Immunodetection for IHC
Substrates for ELISA Detection
Substrates for Western Blot Detection
Activated HRP and HRP Labeling Kit
Anti-HRP Monoclonal Antibody (Part No. MA1-10371)