Streptavidin Agarose and Columns

Thermo Scientific Pierce Streptavidin Agarose is a standard-capacity beaded agarose resin of immobilized recombinant streptavidin protein for use in a variety of biotin-binding affinity purification methods.

Pierce Streptavidin Agarose consists of purified recombinant streptavidin that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. Streptavidin is a tetrameric biotin-binding protein. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin.

Highlights:

• Streptavidin – purified recombinant streptavidin protein (53kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
• Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable – streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• Standard capacity – this variety of beads has a normal load of immobilized streptavidin, providing a binding capacity of 1 to 3mg of biotinylated BSA per mL of resin
• Two formats – available in several bottle sizes of resin slurry and as pre-packed 5mL-capacity spin columns containing 1mL of resin

Applications:

• Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies
• Isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent
• Isolation of cell-surface glycoproteins using biotinylated Concanavalin A
• Recovery of single-stranded biotinylated DNA for dideoxy sequencing
• Streptavidin-coated plates used for direct solid-phase sequencing
• In vitro mutagenesis with streptavidin-coated plates
• DNA fingerprinting with streptavidin-coated plates

Product Details:

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

Properties of crosslinked 6% beaded agarose (CL-6B):

• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1mL/minute (for 1cm diameter column)
• Maximum Linear Velocity: 30cm per hour
• Maximum Pressure: less than 25psi (1.5 bar)

References (all Streptavidin Resins):

1. Nielsen K. H. et al. (2011) Synergistic activation of eIF4A by eIF4B and eIF4G. Nucleic Acids Res. 39, 2678-89.
2. Havel L. S. et al. (2011) Preferential accumulation of N-terminal mutant huntingtin in the nuclei of striatal neurons is regulated by phosphorylation. Hum. Mol. Genet. 20, 1424-37.
3. Caraballo J. C. et al. (2011) Hypoxia increases transepithelial electrical conductance and reduces occludin at the plasma membrane in alveolar epithelial cells via PKC-{zeta} and PP2A pathway. Am J Physiol Lung Cell Mol Physiol. 300, L569-78.
4. Nielsen K. H. et al. (2009) Mechanism of ATP turnover inhibition in the EJC. RNA. 15, 67-75.
5. Lamkanfi M. et al. (2008) Targeted peptidecentric proteomics reveals caspase-7 as a substrate of the caspase-1 inflammasomes. Mol. Cell. Proteomics. 7, 2350-63.

Related Products:

Other Biotin-binding Affinity Resins
Purified Streptavidin and Streptavidin Conjugates
Streptavidin Coated Plates
Pierce Streptavidin Magnetic Beads