Pierce Protein A and Protein G IgG Plus Orientation Kits
Crosslink oriented antibodies to agarose resin to create antigen affinity column.
Thermo Scientific Pierce IgG Plus Orientation Kits use a crosslinker to covalently attach antibody that is bound to Protein A or Protein G agarose resin, thereby creating a reusable affinity column for purification of antigens.
These "Plus" kits provide for more efficient and stable antibody immobilization than the traditional orientation method because they use the NHS-ester crosslinker DSS (disuccinimidyl suberate) rather than the imidoester crosslinker DMP (dimethyl pimelimidate). Several milligrams of IgG can be bound and crosslinked to each 2 ml column, afterwhich the prepared affinity column can be used multiple times for antigen purification using typical binding and elution conditions.
Highlights:
Contaminant-free antigen purification – provides for antigen purification without contamination (co-elution) of antibody
Covalent, leak-resistant antibody immobilization – high-capacity binding of IgG to Protein A or Protein G agarose, followed by efficient crosslinking with DSS crosslinker
High-quality agarose resin – crosslinked 6% beaded agarose with recombinant Protein A or Protein G
Gentle binding and crosslinking conditions – coupling antibody in standard PBS
Oriented immobilization – binds and crosslinks IgG with the antigen-binding sites directed outward, able to capture passing antigen in the mobile phase without steric hindrance
Complete kits – include all the reagents needed to immobilize the antibody
Better than DMP – less antibody leaching than traditional orientation method with DMP crosslinker
Product Details:
Immobilization chemistry of Thermo Scientific IgG Plus Orientation Kits. The crosslinker DSS is used to permanently link antibody that has been bound by immobilized Protein G (or A). Learn more about covalent immobilization methods and about DSS crosslinking chemistry.
References:
Venturi, M., et al. (2000). J. Biol. Chem.275(7), 4734-4742.
