Antibody Fragmentation Kits

Optimized reagents and kits based on immobilized papain, pepsin and ficin proteases to selectively cleave IgG and IgM immunoglobulin molecules into Fab, Fab', F(ab')2 and Fc antibody fragments for use in immunodetection and immunoassay procedures.

Selection Guides – Choose an antibody fragmentation kit:
- See: Antibody Fragmentation Methods (Protein Methods article)
- Tech Tip: Choosing a secondary antibody: A guide to fragment specificity
Protein Methods Library – Learn more about antibody characterization:
- Antibody Fragmentation Methods
- Overview of Antibody Production and Purification
Related Product Categories – Other products of interest:
- Proteases and Protein-Cleaving Reagents
- Fragment-specific Secondary Antibodies


	Fab Preparation Kits Digest human or mouse IgG antibodies with immobilized papain protease and purify Fab fragments using Protein A agarose.


	F(ab')2 Preparation Kits Selectively digest whole human and other IgG antibodies with immobilized pepsin protease to make F(ab')2 fragments that retain antigen binding activity.


	Mouse IgG1 Fab and F(ab')2 Preparation Kit Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.