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Antibody Binding and Elution Buffers

Ensure optimal binding and elutions conditions for almost any affinity application.

Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Highlights:

Ready to use – carefully formulated and prefiltered 1X solutions
Three binding buffers – optimized for Protein A, Protein G or Protein A/G
Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)
Versatile – elution buffers are appropriate for antibody-antigen affinity purification

Item-Specific Features:

Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
IgG Elution Buffer: pH 2.8; amine-based
Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

Related Resources:

Review of antibody purification methods

Related Products:

Antibody Purification Resins and Kits
Antigen Immobilization Resins and Kits

Ordering Information

Product #	Description	Pkg. Size	Instructions	MSDS	CofA	Price
21001	Protein A IgG Binding Buffer Formulation: Proprietary buffer (pH 8) with EDTA and glycerol Sufficient For: Maximum binding of IgG to Protein A resin	1L				$76.00
21007	Protein A IgG Binding Buffer Formulation: Proprietary buffer (pH 8) with EDTA and glycerol Sufficient For: Maximum binding of IgG to Protein A resin	3.75L				$214.00
21019	Protein G IgG Binding Buffer Formulation: Proprietary buffer (pH 5) with sodium azide Sufficient For: Maximum binding of IgG to Protein G resin	1L				$69.00
21011	Protein G IgG Binding Buffer Formulation: Proprietary buffer (pH 5) with sodium azide Sufficient For: Maximum binding of IgG to Protein G resin	3.75L				$203.00
54200	Protein A/G IgG Binding Buffer Formulation: Proprietary buffer (pH 8) with EDTA Sufficient For: Maximum binding of IgG to Protein A/G resin	240mL				$39.00
21004	IgG Elution Buffer Formulation: Proprietary amine-buffer (pH 2.8) Sufficient For: High-yield dissociation of IgG from affinity resins	1L				$71.00
21009	IgG Elution Buffer Formulation: Proprietary amine-buffer (pH 2.8) Sufficient For: High-yield dissociation of IgG from affinity resins	3.75L				$178.00
21028	IgG Elution Buffer, pH 2.0 Formulation: Proprietary amine-buffer (pH 2.0) Sufficient For: high-yield dissociation of IgG from affinity resins	1L				$70.00
21020	Gentle Ag/Ab Binding Buffer, pH 8.0 Formulation: Sodium borate buffer (pH 8) Sufficient For: Phosphate-free binding of IgG for direct compatibility with Gentle Ag/Ab Elution Buffer	1L				$108.00
21012	Gentle Ag/Ab Binding Buffer, pH 8.0 Formulation: Sodium borate buffer (pH 8) Sufficient For: Phosphate-free binding of IgG for direct compatibility with Gentle Ag/Ab Elution Buffer	3.75L				$206.00
21027	Gentle Ag/Ab Elution Buffer, pH 6.6 Formulation: Proprietary, high-molarity buffer (pH 6.55) Sufficient For: Near-neutral elution of IgG	500mL				$97.00
21013	Gentle Ag/Ab Elution Buffer, pH 6.6 Formulation: Proprietary, high-molarity buffer (pH 6.55) Sufficient For: Near-neutral elution of IgG	3.75L				$290.00
21013B	Gentle Ag/Ab Elution Buffer, pH 6.6 Formulation: Proprietary, high-molarity buffer (pH 6.55) Sufficient For: Near-neutral elution of IgG	Custom
21030	Gentle Ag/Ab Binding and Elution Buffer Kit Formulation: Multi-component kit Sufficient For: Binding and near-neutral elution of antibodies with Protein A or G Kit Contents: Pierce Gentle Ag/Ab Binding Buffer, 100mL Pierce Gentle Ag/Ab Elution Buffer, 100mL	200mL-kit				$103.00