Nitrocellulose-coated glass slides for protein microarray development.

The PATH* Protein Microarray Slide features an ultra-thin nitrocellulose coating for the non-covalent, yet irreversible, capture of active proteins to the surface. Nitrocellulose is a well-known and proven platform for immunoassay applications, including tissue lysate, antigen, direct and sandwich ELISA arrays. The PATH Slide is the first protein microarray slide to provide the many benefits of nitrocellulose in a thin film that allows very low background fluorescence and excellent signal-to-noise ratios.

The PATH Slide can be used for a variety of assays, such as sandwich immunoassays, capture immunoassays, protein profiling, and protein characterization. Guidelines are provided for a typical fluorescence-based sandwich immunoassay; however, optimization may be necessary for specific experimental systems. These slides are provided ready to use and contain an etched barcode and accompanying numbers that allows easy identification of the printable side.

Highlights:

• Low background and increased dynamic range – proprietary ultra-thin nitrocellulose offers enhanced signal-to-noise ratios
• Excellent stability and shelf life – ambient shipping and storage
• Standard slide format – works with all commonly available slide format microarray readers
• Individual bar-coding – easy identification for reliable tracking and documentation for improved experimental and quality control

Applications for Nitrocellulose-coated Glass Slides:

PATH Technology consists of a proprietary ultra-thin nitrocellulose film to bind antibodies non-covalently and irreversibly to the surface. The result is high sensitivity for antibody microarray applications. Nitrocellulose is a well-known and proven platform for immunoassay applications. Applications include:

• Quantitative multiplex immunoassays
• Multiplex serological assays
• Single capture antibody microarrays
• Biomarker profiling
• Protein microarrays are used in all phases of drug development

Product Specifications:

• Nitrocellulose thickness < 500 nm
• Open platform – usable surface area is 58 x 21 mm
• Slide dimensions – 75 x 25 x 1 mm
• Unique bar code

	vs
PATH Nitrocellulose Slide		Conventional Nitrocellulose Slide

Low fluorescence coating provides better performance for fluorescent microarray assays. Fluorescence scans of blank PATH Protein Microarray Slide and conventional nitrocellulose slide illustrates an advantage of ultra-thin nitrocellulose films. Blank slides were scanned using identical parameters (543 nm Cy3* Fluor setting; Scanarray* 4000 Imager (PMT=90. Gain=75); QuantArray* Software; 500 evenly spaced circles.

• PATH Slide:
  • Mean RFU: 398
  • Std. Dev.: 15
  • %C.V.: 3.07
• Conventional Slide:
  • Mean RFU: 24998
  • Std. Dev.: 1517
  • %C.V.: 6.07

Related Products:
Other Microarray Glass Slides – aminopropyltriethoxysilane-, epoxysilane-, aldehyde-coated
ExcelArray Antibody Sandwich Microarray Kits – multiplex cytokine assays